Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Can IDT custom synthesize a dual-labeled oligonucleotide (5' FAM/3' TAMRA)?

IDT can synthesize dual-labeled probes on several scales with a variety of fluorophores and quenchers including 5' FAM and 3' TAMRA. Scales range from "mini" up through a custom large scale request. In addition, a second quencher (ZEN Quencher) can be added to decrease background and increase signal. More information on IDT's dual-labeled probe options can be found at the following link: www.idtdna.com/pages/products/gene-expression/primetime-qpcr-probes-and-quenchers.