Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Can the PrimeTime® Gene Expression Master Mix be used with fast cycling conditions?

Yes, it can be used with either standard or fast cycling conditions. The conditions for both are provided below, and can also be found in the PrimeTime Gene Expression Master Mix protocol.

  • Standard cycling conditions: 3 min. 95°C; 35–45 x (15 sec. 95°C; 1 min. 60°).
  • Fast cycling conditions: 3 min. 95°C; 35–45 x (5 sec. 95°C; 30 sec. 60°).

Categories:
qPCR & PCR