Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Do I need to form my gRNA/RNP complexes separately before multiplexing?

Yes. Forming the crRNA:tracrRNA:Cas9 ribonucleoprotein complex prior to mixing multiple crRNAs helps prevent undesireable interactions between different crRNAs before they have an opportunity to correctly hybridize with tracrRNA.