Frequently asked questions

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How do I resuspend my PrimeTime® qPCR Assay?

PrimeTime qPCR Assays can be re-suspended as 40X, 20X, or 10X stocks. Different concentrations may be preferred depending on the size of the assay and desired final reaction volume. Resuspend the assay in IDTE Buffer (10 mM Tris, 0.1 mM EDTA pH 8.0—available from IDT) at the volumes indicated in the chart below. After adding the volume indicated, pipette the solution up and down the sides of the tube to ensure maximal product recovery. Following resuspension, store at –20°C.aa_resuspending-primetime-assays