Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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If ZEN™ is positioned so close to FAM, why is the 3’ quencher required?

In many cases, the ZEN™ quencher is the predominant method for dye quenching. However, adding the 3'-IBFQ (Iowa Black Fluorescent Quencher) further reduces background and increases performance. In addition, there may be some sequences that favor the 3' quencher due to oligo folding when not annealed to a complementary sequence.

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qPCR & PCR