Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Is there a difference between the streptavidin binding affinity of 3’ and 5' biotin modifications?

Our 3' and 5' biotin modifications have the same functional biotin molecule and bind streptavidin with equal affinity. A 3' biotin modification will block extension, so 5' biotin should be selected if your oligo will be used in an extension reaction. Read more about our biotin modifications and their recommended applications in the DECODED newsletter article, Which Biotin Modification to Use?

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