Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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What is the optimal length of a primer?

IDT recommends you aim for primers between 18–30 bases; however the most important considerations for primer design should be their Tm value and specificity. Primers should also be free of strong secondary structures and self-complementarity. The free IDT OligoAnalyzer SciTool can be used to evaluate these features.