Frequently asked questions

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What is the protospacer adjacent motif (PAM) sequence for Cpf1? How is this different from Cas9?

The PAM sequence for the Cpf1 system is TTTV [1], where "V" is a A, C, or G. The Cpf1 PAM sequence can be advantageous when working with T-rich target sequences. In contrast, the Cas9 PAM sequence is NGG. 

Reference

  1. Zetsche B, Gootenberg JS, et al. (2015) Cpf1 is a single RNA-guided endonuclease of a Class 2 CRISPR-Cas system. Cell, 163(3):759–771.