Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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When preparing the qPCR reaction mix, should I thaw or keep reagents (such as the PrimeTime® Gene Expression Master Mix) on ice?

It is good practice, but not necessary to thaw or keep reagents on ice. We see no difference in performance between reaction mixes prepared with the PrimeTime® Gene Expression Master Mix that were used immediately vs those kept at room temperature for up to 24 hours. PrimeTime Gene Expression Master Mix also shows exceptional temperature stability, even after a heat stress test (up to 8 hr at 55°C). Review the data.

Categories:
qPCR & PCR