Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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When should I choose PAGE vs HPLC purification?

IDT recommends PAGE purification for long unmodified oligos and oligos used in cloning, gel shift, and mutagenesis protocols. Due to their hydrophobicity, most oligos with modifications are better separated by HPLC. Therefore, IDT requires HPLC purification of oligos with specific modifications. HPLC routinely provides 85% purity, while PAGE provides 90% purity. The final yield of a PAGE purified oligo, however, will be less than an HPLC purified oligo. For more details on our purification services, click here.