Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Where on a nucleotide are Cy dyes attached?

5′ and 3′ Cy dyes are attached to the hydroxyl group of the ribose via a phosphodiester bond. Internal Cy dyes are attached to the backbone via the phosphodiester bonds of the bases between which the dyes occur.