Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Which negative control Dicer-substrate siRNA (DsiRNA) should I choose, DS NC1 or DS ScrambledNeg?

We currently offer 2 nonsilencing, negative control DsiRNAs that do not recognize any sequences in human, mouse, or rat transcriptomes, a nontargeting DsiRNA (DS NC1) and a DsiRNA with a scrambled sequence (DS ScrambledNeg). We strongly recommend using the DS NC1 negative control duplex. This has been extensively bioinformatically screened, and has no activity in human, mouse, and rat cells. The DS ScrambledNeg sequence is an older control that has been kept as a legacy product for customers who have had success with it in their cell lines. Learn more and order from the Dicer-Substrate siRNA (DsiRNA) page.