Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Why did IDT decide to ship PrimeTime® Gene Expression Master Mix at ambient temperature?

We tested PrimeTime master mix and demonstrated consistent qPCR performance in singleplex and duplex reactions after the master mix had been exposed to extreme temperature conditions (50°C for up to 7 days; up to 20 freeze-thaw cycles). These treatments exceed conditions experienced by the master mix during routine shipping or laboratory use. The reliable performance of the master mix allows for shipping at ambient temperature, providing environmental benefits and research dollar savings.

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qPCR & PCR