TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Modification Highlight: Modifications that Block Nuclease Degradation

Description of modifications that can be added to an oligo to limit nuclease degradation, for example, when experiments where the oligos are to be used in cell culture or in vivo.

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CRISPR and Cas9 for Flexible Genome Editing

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) are sequences that serve as an adaptive defense ("immune") systems in bacteria and archaea . In conjunction with CRISPR associated (Cas) proteins, CRISPR sequences are able to recognize and cleave complementary DNA sequences. This natural mechanism has been coopted by scientists for targeted gene editing or removal. This article describes some of the early applications for which this technology is being used.

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Digital PCR (dPCR)—What Is It and Why Use It?

General overview of dPCR and how it can be used for qPCR applications, including multiplex qPCR.

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Modification Highlight: When dT is Required For Modification Attachment

Certain modifications require a dT base in the oligonucleotide sequence in order to be added.

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Exon Numbering—Not As Easy As 1, 2, 3...

Exon numbering and location data can differ across various software tools, including with NCBI's gene database. For example, exons within alternatively spliced transcripts are sometimes individually numbered, with no consistent gene-based numbering system across these transcripts for identifying exons. Here we describe how IDT exon location information gives each exon a unique number and how that compares with the NCBI naming system.

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