TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Oligo synthesis: Why IDT leads the oligo industry

Read about the phosphoramidite method of oligonucleotide synthesis that IDT uses in its manufacturing processes. We also highlight the additional measures we take to ensure our customers receive the highest quality oligos and nucleic acid products in the shortest time possible.

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Antisense Oligonucleotides (ASOs)

Modification Highlight: The first oligonucleotide-based approach for disrupting gene expression—learn about new applications for antisense oligos, including the study of lncRNA function.

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Tips for successful lncRNA knockdown: Design, delivery, and analysis of antisense and RNAi reagents

IDT research scientist Kim Lennox has been optimizing effective lncRNA knockdown with antisense and RNAi reagents. Here she provides some tips for successful lncRNA knockdown.

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A new renaissance for antisense in the era of lncRNA

Noncoding RNAs such as lncRNA, are much more prevalent in humans than protein-coding RNA. Antisense oligonucleotides (ASO), previously used for knockout experiments, are being employed to study the role of noncoding RNAs in gene regulation. ASOs provide several advantages over siRNAs (and DsiRNA) for this purpose.

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Site-directed mutagenesis—improvements to established methods

Site-directed mutagenesis techniques have relied primarily on PCR and standard cloning methods. Read about some of the common cloning methods used for mutagenesis and how double-stranded DNA fragments (gBlocks Gene Fragments) can save you both time and money.

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