TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

A new renaissance for antisense in the era of lncRNA

Noncoding RNAs such as lncRNA, are much more prevalent in humans than protein-coding RNA. Antisense oligonucleotides (ASO), previously used for knockout experiments, are being employed to study the role of noncoding RNAs in gene regulation. ASOs provide several advantages over siRNAs (and DsiRNA) for this purpose.

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Site-directed mutagenesis—improvements to established methods

Site-directed mutagenesis techniques have relied primarily on PCR and standard cloning methods. Read about some of the common cloning methods used for mutagenesis and how double-stranded DNA fragments (gBlocks Gene Fragments) can save you both time and money.

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Need a non-standard modification?

Not finding the modification you need? IDT will consider any modification you have in mind. In addition to our standard modifications, IDT offers 89 modifications that are not listed in our online catalog. A few of the more popular ones are described along with information on how to order them. Contact us with any inquiries at noncat@idtdna.com.

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Interpreting Melt Curves: An Indicator, Not a Diagnosis

Examining PCR melt curve data to determine what it can/cannot tell us about resulting PCR amplicons.

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Target enrichment facilitates focused next generation sequencing

The rationale and benefits of enriching subsets of the genome (target enrichment by hybrid capture) prior to sequencing.

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