Interpreting melt curves: An indicator, not a diagnosis

Performing intercalating dye PCR/qPCR assays? Review examples of PCR melt curve data with our scientists to determine what it can/cannot tell us about resulting PCR amplicons.

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Target enrichment facilitates focused next generation sequencing

The rationale and benefits of enriching subsets of the genome (target enrichment by hybrid capture) prior to sequencing.

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Modifications that block nuclease degradation

Modification Highlight: Are you working with your oligos in cells culture or in vivo? Find out which modifications can be added to an oligo to limit nuclease degradation.

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CRISPR and Cas9 for flexible genome editing

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) are sequences that serve as an adaptive defense ("immune") systems in bacteria and archaea. Learn how scientists have coopted this natural mechanism for targeted gene editing or removal. This article also describes some of the early applications for which this technology is being used.

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Digital PCR (dPCR)—What is it and why use it?

General overview of dPCR and how it can be used for qPCR applications, including multiplex qPCR.

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