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Digital PCR (dPCR)—What is it and why use it?

General overview of dPCR and how it can be used for qPCR applications, including multiplex qPCR.

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When dT is required for modification attachment

Modification Highlight: Certain modifications require a dT base in the oligonucleotide sequence in order to be added.

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Exon numbering—not as easy as 1, 2, 3...

Exon numbering and location data can differ across various software tools, including with NCBI's gene database. For example, exons within alternatively spliced transcripts are sometimes individually numbered, with no consistent gene-based numbering system across these transcripts for identifying exons. Learn how IDT exon location information gives each exon a unique number and how that compares with the NCBI naming system.

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How to avoid false positives in PCR and what to do if you get them

Causes of false positives in the Negative Template Control sample during PCR, and suggestions for preventing them.

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Using antisense technologies to modulate noncoding RNA function

Review useful modifications and design considerations for effective antisense oligonucleotides.

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