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CRISPR and Cas9 for flexible genome editing

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) are sequences that serve as an adaptive defense ("immune") systems in bacteria and archaea. Learn how scientists have coopted this natural mechanism for targeted gene editing or removal. This article also describes some of the early applications for which this technology is being used.

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Digital PCR (dPCR)—What is it and why use it?

Read this general summary of dPCR, and how it can be used for qPCR applications, including multiplex qPCR.

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When dT is required for modification attachment

Modification Highlight: Certain modifications require a dT base in the oligonucleotide sequence in order to be added. Learn which modifications these are, and how to add them to your oligo sequence.

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Exon numbering—not as easy as 1, 2, 3...

Exon numbering and location data can differ across various software tools, including with NCBI's gene database. For example, exons within alternatively spliced transcripts are sometimes individually numbered, with no consistent gene-based numbering system across these transcripts for identifying exons. Learn how IDT exon location information gives each exon a unique number and how that compares with the NCBI naming system.

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How to avoid false positives in PCR and what to do if you get them

Do you know what causes false positives in the Negative Template Control sample during PCR? Review the causes and get these suggestions for preventing them.

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