When dT is required for modification attachment

Modification Highlight: Certain modifications require a dT base in the oligonucleotide sequence in order to be added.

Read More>

Exon numbering—not as easy as 1, 2, 3...

Exon numbering and location data can differ across various software tools, including with NCBI's gene database. For example, exons within alternatively spliced transcripts are sometimes individually numbered, with no consistent gene-based numbering system across these transcripts for identifying exons. Learn how IDT exon location information gives each exon a unique number and how that compares with the NCBI naming system.

Read More>

When designing PCR assays, always check for SNPs

With the increasing identification of SNPs, it is critical to check whether they underlie your primer or probe sequences, which could compromise PCR efficiency.

Read More>

How to Avoid False Positives in PCR and What to Do If You Get Them

Causes of false positives in the Negative Template Control sample during PCR, and suggestions for preventing them.

Read More>

Using antisense technologies to modulate noncoding RNA function

Review useful modifications and design considerations for effective antisense oligonucleotides.

Read More>


Get a free, ongoing print or email subscription to the DECODED newsletter by registering online.