TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

G Repeats—Structural Challenges for Oligo Design

What are G-quadruplexes and how do they affect oligonucleotide synthesis and applications?

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DNA Oligonucleotide Resuspension and Storage

Upon receiving newly synthesized oligonucleotides, researchers must decide how to resuspend and store the product. Here are some guidelines and recommendations.

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Cloning Strategies Part 1: Assembly PCR for Novel Gene Synthesis

Single-stranded oligos or a mix of single- and double-stranded DNA are used to produce longer genes of up to several thousand bp.

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Oligo Modification—Post-Synthesis Conjugation Explained

Addition of NHS esters, and of Amino and Alkene mods through Click Chemistry. Common questions regarding post-synthesis conjugation are also answered.

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Which Biotin Modification to Use?

Applications of each of the different biotins available from IDT.

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