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TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Assembly PCR for Novel Gene Synthesis

Single-stranded oligos or a mix of single- and double-stranded DNA are used to produce longer genes of up to several thousand base pairs.

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Oligo Modification—Post-Synthesis Conjugation Explained

Addition of NHS esters, and of Amino and Alkene mods through Click Chemistry. Common questions regarding post-synthesis conjugation are also answered.

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Which Biotin Modification to Use?

Applications of each of the different biotins available from IDT.

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Modification Highlight: Digoxigenin

Digoxigenin—a modification suitable for use as an alternative to biotin/streptavidin and in non-radioactive hybridization applications.

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qPCR Terminology—What Does It Mean?

Definitions of some of the most commonly used terms and distinctions encountered in qPCR experiments.

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