TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Target enrichment facilitates focused next generation sequencing

Understand the rationale and benefits of enriching subsets of the genome (target enrichment by hybrid capture) prior to sequencing. Use this strategy for genotyping, identifying splice variants and indels, and profiling genomic recombination events as well as viral and transposon integration sites.

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Oligonucleotide modifications that block nuclease degradation

Modification Highlight: Are you working with your oligos in cells culture or in vivo? Find out which modifications can be added to an oligo to limit nuclease degradation.

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CRISPR and Cas9 for flexible genome editing

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) are sequences that serve as an adaptive defense ("immune") systems in bacteria and archaea. Learn how scientists have coopted this natural mechanism for targeted gene editing or removal. This article also describes some of the early applications for which this technology is being used.

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Digital PCR (dPCR)—What is it and why use it?

Read this general summary of dPCR, and how it can be used for qPCR applications, including multiplex qPCR.

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When dT is required for modification attachment

Modification Highlight: Certain modifications require a dT base in the oligonucleotide sequence in order to be added. Learn which modifications these are, and how to add them to your oligo sequence.

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