ZEN™ Internal Quencher

Modification Highlight: Use of the ZEN Quencher as a second, internal quencher in qPCR 5’-nuclease assay probes provides greater overall dye quenching, lowering background, and increasing signal detection. When incorporated into oligonucleotides, it also serves to strengthen duplex formation and block exonuclease digestion, while remaining nontoxic to cells. Thus the ZEN Quencher can be useful in steric blocking antisense oligonucleotide applications.

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Multiplex qPCR—how to get started

Learn how multiplex qPCR can save sample, reagent cost, and time. The article provides recommendations for multiplex qPCR assay design and experimental setup.

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Oligos for molecular diagnostics

Understand the difference between Good Manufacturing Practices (GMP) and ISO 13485 certification and what these credentials mean for oligonucleotides manufactured for human diagnostics.

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G repeats—structural challenges for oligo design

Find out what G-quadruplexes are and how they can affect oligonucleotide synthesis and applications.

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DNA Oligonucleotide Resuspension and Storage

Upon receiving newly synthesized oligonucleotides, researchers must decide how to resuspend and store the product. Here are some guidelines and recommendations.

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