When dT is required for modification attachment

Modification Highlight: Certain modifications require a dT base in the oligonucleotide sequence in order to be added. Learn which modifications these are, and how to add them to your oligo sequence.

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Exon numbering—not as easy as 1, 2, 3...

Exon numbering and location data can differ across various software tools, including with NCBI's gene database. For example, exons within alternatively spliced transcripts are sometimes individually numbered, with no consistent gene-based numbering system across these transcripts for identifying exons. Learn how IDT exon location information gives each exon a unique number and how that compares with the NCBI naming system.

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How to avoid false positives in PCR and what to do if you get them

Do you know what causes false positives in the Negative Template Control sample during PCR? Review the causes and get these suggestions for preventing them.

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Using antisense technologies to modulate noncoding RNA function

RNase H–dependent antisense silencing using gapmer ASOs is a powerful tool for specific modulation of nuclear noncoding RNAs. Review these useful modifications and design considerations for effective antisense oligonucleotides.

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Better PCR probes: A second quencher lowers background, increasing signal detection

Modification Highlight: Add the ZEN Quencher as a second, internal quencher in qPCR 5’-nuclease assay probes to obtain greater overall dye quenching, lowering background, and increasing signal detection. When incorporated into oligonucleotides, it also serves to strengthen duplex formation and block exonuclease digestion, while remaining nontoxic to cells. Thus the ZEN Quencher can be useful in steric blocking antisense oligonucleotide applications.

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