TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

How to avoid false positives in PCR and what to do if you get them

Do you know what causes false positives in the Negative Template Control sample during PCR? Review the causes and get these suggestions for preventing them.

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Using antisense technologies to modulate noncoding RNA function

Review useful modifications and design considerations for effective antisense oligonucleotides.

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Better PCR probes: A second quencher lowers background, increasing signal detection

Modification Highlight: Add the ZEN Quencher as a second, internal quencher in qPCR 5’-nuclease assay probes to obtain greater overall dye quenching, lowering background, and increasing signal detection. When incorporated into oligonucleotides, it also serves to strengthen duplex formation and block exonuclease digestion, while remaining nontoxic to cells. Thus the ZEN Quencher can be useful in steric blocking antisense oligonucleotide applications.

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Oligos for molecular diagnostics

Understand the difference between Good Manufacturing Practices (GMP) and ISO 13485 certification and what these credentials mean for oligonucleotides manufactured for human diagnostics.

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G repeats—structural challenges for oligo design

G-quadruplexes are formed from the stacking of two or more G-tetrads, which occur naturally in sequences with short G base repeats. Find out more about G-quadruplexes and how they can affect oligonucleotide synthesis and applications.

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