TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Oligos for molecular diagnostics

Understand the difference between Good Manufacturing Practices (GMP) and ISO 13485 certification and what these credentials mean for oligonucleotides manufactured for human diagnostics.

Read More>

G repeats—structural challenges for oligo design

Find out what G-quadruplexes are and how they can affect oligonucleotide synthesis and applications.

Read More>

DNA Oligonucleotide Resuspension and Storage

You just received your newly synthesized oligonucleotides. Now what? Here are some guidelines and recommendations on how to resuspend and store your oligos.

Read More>

Cloning strategies, Part 1: Assembly PCR for novel gene synthesis

Learn how you can use single-stranded oligos or a mix of single- and double-stranded DNA to produce longer genes of up to several thousand base pairs. No restriction sites are needed, and the approach is beneficial for assembling constructs that contain modular elements, such as antibodies.

Read More>

Oligo Modification—Post-Synthesis Conjugation Explained

Addition of NHS esters, and of Amino and Alkene mods through Click Chemistry. Common questions regarding post-synthesis conjugation are also answered.

Read More>

SUBSCRIBE

Get a free, ongoing print or email subscription to the DECODED newsletter by registering online.

DECODED ARCHIVES