TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Oligonucleotide Modifications: Choosing the Right Mod for Your Needs

Guidelines on selecting oligonucleotide modifications and how they can help you in your research.

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The Importance of Tm in Molecular Biology Applications

Predicting and selecting appropriate Tms for oligo hybridization steps (e.g. for PCR).

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Cloning Strategies Part 2: Cohesive-End Cloning

Tips and tricks for cloning using restriction enzymes.

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Isothermal Assembly: Quick, Easy Gene Construction

In a single reaction, isothermal assembly combines several overlapping DNA fragments to produce a ligated plasmid ready for transformation.

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Modification Highlight: Photo-Cleavable Spacer

How photocleavable spacers can be used in your research.

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