Cloning Strategies Part 3: Blunt-End Cloning

Blunt-end cloning is one of the easiest and most versatile methods for cloning dsDNA into plasmid vectors. It is easy because the blunt-ended insert requires little to no preparation. Read an overview of blunt-end cloning with tips for making this cloning approach successful.

Read More>

Photo-Cleavable Spacer

Modification Highlight: This modification can be cleaved by a specific wavelength of UV light, fragmenting an oligo or releasing a terninal modification such as a fluorophore.

Read More>

Oligonucleotide modifications: Choosing the right mod for your needs

Guidelines on selecting oligonucleotide modifications and how they can help you in your research.

Read More>

The Importance of Tm in Molecular Biology Applications

Predicting and selecting appropriate Tms for oligo hybridization steps (e.g. for PCR).

Read More>

Cloning Strategies Part 2: Cohesive-End Cloning

Tips and tricks for cloning using restriction enzymes.

Read More>


Get a free, ongoing print or email subscription to the DECODED newsletter by registering online.