TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Cloning strategies, Part 1: Assembly PCR for novel gene synthesis

Learn how you can use single-stranded oligos or a mix of single- and double-stranded DNA to produce longer genes of up to several thousand base pairs. No restriction sites are needed, and the approach is beneficial for assembling constructs that contain modular elements, such as antibodies.

Read More>

Oligo modification—post-synthesis conjugation explained

Did you know IDT can add modifications to your oligos post-synthesis using NHS Ester chemistry? We can also add modifications through azide and alkyne groups post-synthesis, using click chemistry. Read about how these reactions are done, and get answers to common questions regarding post-synthesis conjugation.

Read More>

Which Biotin Modification to Use?

Applications of each of the different biotins available from IDT.

Read More>

A biotin:streptavidin alternative for non-radioactive hybridization assays—Digoxigenin

Modification Highlight: Learn about Digoxigenin—a modification suitable for use as an alternative to biotin/streptavidin for non-radioactive hybridization applications.

Read More>

qPCR terminology—what does it mean?

Review these definitions of some of the most commonly used terms and distinctions encountered in qPCR experiments.

Read More>

SUBSCRIBE

Get a free, ongoing print or email subscription to the DECODED newsletter by registering online.

DECODED ARCHIVES