TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

DNA Oligonucleotide Resuspension and Storage

You just received your newly synthesized oligonucleotides. Now what? Here are some guidelines and recommendations on how to resuspend and store your oligos.

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Cloning strategies, Part 1: Assembly PCR for novel gene synthesis

Learn how you can use single-stranded oligos or a mix of single- and double-stranded DNA to produce longer genes of up to several thousand base pairs. No restriction sites are needed, and the approach is beneficial for assembling constructs that contain modular elements, such as antibodies.

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Oligo modification—post-synthesis conjugation explained

Did you know IDT can add modifications to your oligos post-synthesis using NHS Ester chemistry? We can also add modifications through azide and alkyne groups post-synthesis, using click chemistry. Read about how these reactions are done, and get answers to common questions regarding post-synthesis conjugation.

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Which biotin modification to use?

Biotin is important in many kinds of molecular biology applications, in part due to its very high affinity for streptavidin and avidin. It is used in mobility shift assays, and for enrichment, purification, and attachment to solid surfaces. Biotin can also be used for tagging target molecules with dye- or enzyme-labeled streptavidin. But did you know there are numerous forms of biotin that can be used for such applications? Read about the differences between Standard Biotin, Biotin dT, Biotin-TEG. Dual Biotin, Photocleavable Biotin, DesthioBiotin-TEG, and Biotin Azide.

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A biotin:streptavidin alternative for non-radioactive hybridization assays—Digoxigenin

Modification Highlight: Learn about Digoxigenin—a modification suitable for use as an alternative to biotin/streptavidin for non-radioactive hybridization applications.

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