TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Oligo Modification—Post-Synthesis Conjugation Explained

Addition of NHS esters, and of Amino and Alkene mods through Click Chemistry. Common questions regarding post-synthesis conjugation are also answered.

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Which Biotin Modification to Use?

Applications of each of the different biotins available from IDT.

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A biotin:streptavidin alternative for non-radioactive hybridization assays—Digoxigenin

Modification Highlight: Learn about Digoxigenin—a modification suitable for use as an alternative to biotin/streptavidin for non-radioactive hybridization applications.

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qPCR terminology—what does it mean?

Review these definitions of some of the most commonly used terms and distinctions encountered in qPCR experiments.

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Cloning Strategies Part 3: Blunt-End Cloning

Blunt-end cloning is one of the easiest and most versatile methods for cloning dsDNA into plasmid vectors. It is easy because the blunt-ended insert requires little to no preparation. Read an overview of blunt-end cloning with tips for making this cloning approach successful.

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