TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Oligos for molecular diagnostics

Understand the difference between Good Manufacturing Practices (GMP) and ISO 13485 certification and what these credentials mean for oligonucleotides manufactured for human diagnostics.

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G repeats—structural challenges for oligo design

G-quadruplexes are formed from the stacking of two or more G-tetrads, which occur naturally in sequences with short G base repeats. Find out more about G-quadruplexes and how they can affect oligonucleotide synthesis and applications.

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DNA Oligonucleotide Resuspension and Storage

You just received your newly synthesized oligonucleotides. Now what? Here are some guidelines and recommendations on how to resuspend and store your oligos.

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Cloning strategies, Part 1: Assembly PCR for novel gene synthesis

Learn how you can use single-stranded oligos or a mix of single- and double-stranded DNA to produce longer genes of up to several thousand base pairs. No restriction sites are needed, and the approach is beneficial for assembling constructs that contain modular elements, such as antibodies.

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Oligo modification—post-synthesis conjugation explained

Did you know IDT can add modifications to your oligos post-synthesis using NHS Ester chemistry? We can also add modifications through azide and alkyne groups post-synthesis, using click chemistry. Read about how these reactions are done, and get answers to common questions regarding post-synthesis conjugation.

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