Cloning Strategies Part 3: Blunt-End Cloning

Blunt-end cloning is one of the easiest and most versatile methods for cloning dsDNA into plasmid vectors. It is easy because the blunt-ended insert requires little to no preparation. Read an overview of blunt-end cloning with tips for making this cloning approach successful.

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Modification Highlight: This modification can be cleaved by a specific wavelength of UV light, fragmenting an oligo or releasing a terminal modification, such as a fluorophore.

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Oligonucleotide modifications: Choosing the right mod for your needs

Guidelines on selecting oligonucleotide modifications and how they can help you in your research.

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The Importance of Tm in Molecular Biology Applications

Predicting and selecting appropriate Tms for oligo hybridization steps (e.g. for PCR).

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Cloning Strategies Part 2: Cohesive-End Cloning

Tips and tricks for cloning using restriction enzymes.

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