TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Which biotin modification to use?

Biotin is important in many kinds of molecular biology applications, in part due to its very high affinity for streptavidin and avidin. It is used in mobility shift assays, and for enrichment, purification, and attachment to solid surfaces. Biotin can also be used for tagging target molecules with dye- or enzyme-labeled streptavidin. But did you know there are numerous forms of biotin that can be used for such applications? Read about the differences between Standard Biotin, Biotin dT, Biotin-TEG. Dual Biotin, Photocleavable Biotin, DesthioBiotin-TEG, and Biotin Azide.

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A biotin:streptavidin alternative for non-radioactive hybridization assays—Digoxigenin

Modification Highlight: Learn about Digoxigenin—a modification suitable for use as an alternative to biotin/streptavidin for non-radioactive hybridization applications.

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qPCR terminology—what does it mean?

Review these definitions of some of the most commonly used terms and distinctions encountered in qPCR experiments.

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Cloning strategies, Part 3: Blunt-end cloning

Blunt-end cloning is one of the easiest and most versatile methods for cloning dsDNA into plasmid vectors. It is easy because the blunt-ended insert requires little to no preparation. Read an overview of blunt-end cloning with tips for making this cloning approach successful.

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Photo-cleavable spacer

Modification Highlight: This modification can be cleaved by a specific wavelength of UV light, fragmenting an oligo or releasing a terminal modification, such as a fluorophore.

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DECODED ARCHIVES