TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Oligonucleotide modifications: Choosing the right mod for your needs

Learn about our broad family of oligonucleotide modifications, and get suggestions for selecting modifications that can help you in your research.

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The importance of Tm in molecular biology applications

Learn how to predict and select appropriate Tms for oligo hybridization steps, including PCR.

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Cloning strategies, Part 2: Cohesive-end cloning

Cohesive-end cloning is one of the most commonly employed techniques in molecular biology. Review these tips and tricks for cloning using restriction enzymes.

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Isothermal Assembly: Quick, Easy Gene Construction

In a single reaction, isothermal assembly combines several overlapping DNA fragments to produce a ligated plasmid ready for transformation.

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Methods for Site-Directed Mutagenesis

Overview of the main techniques used for mutating specific gene construct regions.

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