TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Photo-cleavable spacer

Modification Highlight: This modification can be cleaved by a specific wavelength of UV light, fragmenting an oligo or releasing a terminal modification, such as a fluorophore.

Read More>

Oligonucleotide modifications: Choosing the right mod for your needs

Guidelines on selecting oligonucleotide modifications and how they can help you in your research.

Read More>

The importance of Tm in molecular biology applications

Learn how to predict and select appropriate Tms for oligo hybridization steps, including PCR.

Read More>

Cloning Strategies Part 2: Cohesive-End Cloning

Tips and tricks for cloning using restriction enzymes.

Read More>

Isothermal Assembly: Quick, Easy Gene Construction

In a single reaction, isothermal assembly combines several overlapping DNA fragments to produce a ligated plasmid ready for transformation.

Read More>

SUBSCRIBE

Get a free, ongoing print or email subscription to the DECODED newsletter by registering online.

DECODED ARCHIVES