TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

The Importance of Tm in Molecular Biology Applications

Predicting and selecting appropriate Tms for oligo hybridization steps (e.g. for PCR).

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Cloning Strategies Part 2: Cohesive-End Cloning

Tips and tricks for cloning using restriction enzymes.

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Isothermal Assembly: Quick, Easy Gene Construction

In a single reaction, isothermal assembly combines several overlapping DNA fragments to produce a ligated plasmid ready for transformation.

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Methods for Site-Directed Mutagenesis

Overview of the main techniques used for mutating specific gene construct regions.

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Phosphorothioates

Modification Highlight: Phosphorothioate bonds substitute a sulfur atom for one of the non-bridging oxygen atoms in the phosphate backbone of an oligonucleotide.

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