TECHVAULT > DECODED ARCHIVE > CORE CONCEPTS

Oligonucleotide modifications: Choosing the right mod for your needs

Learn about our broad family of oligonucleotide modifications, and get suggestions for selecting modifications that can help you in your research.

Read More>

The importance of Tm in molecular biology applications

Learn how to predict and select appropriate Tms for oligo hybridization steps, including PCR.

Read More>

Cloning strategies, Part 2: Cohesive-end cloning

Cohesive-end cloning is one of the most commonly employed techniques in molecular biology. Review these tips and tricks for cloning using restriction enzymes.

Read More>

Isothermal assembly: Quick, easy gene construction

Learn how, in a single reaction, isothermal assembly can combine several overlapping DNA fragments to produce a ligated plasmid ready for transformation.

Read More>

Methods for site-directed mutagenesis

Review these traditional PCR-based methods for creating a specific mutation in a known sequence, in vitro. Then read our follow-up article, Site-directed mutagenesis—Improvements to established methods (see the "Additional reading" sidebar) which describes how you can generate the same types of mutations, more quickly and efficiently, using custom, synthetic dsDNA fragments.

Read More>

DECODED ARCHIVES