Methods for Site-Directed Mutagenesis

Overview of the main techniques used for mutating specific gene construct regions.

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Increase oligo stability with phosphorothioate modifications

Modification Highlight: Phosphorothioate bonds substitute a sulfur atom for one of the non-bridging oxygen atoms in the phosphate backbone of an oligonucleotide. Resistant to both endo- and exonucleases, this linkage provides increased oligo stability.

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Starting with RNA—one‑step or two‑step RT‑qPCR?

When performing real-time qPCR, one has to decide whether to use a one-step protocol that combines the RT reaction and PCR in one tube, or a two-step protocol where the RT reaction is performed separately from the PCR.

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RNAi and DsiRNA: Pathway, Mechanism, and Design

An overview of the RNA interference (RNAi) pathway, its applications, and design considerations for gene silencing experiments.

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Time to go GMP?

Learn how GMP manufacture of oligos for diagnostic use provides customers defined specifications and control over manufacturing processes.

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