RNAi and DsiRNA: Pathway, Mechanism, and Design

An overview of the RNA interference (RNAi) pathway, its applications, and design considerations for gene silencing experiments.

Read More>

Time to go GMP?

Learn how GMP manufacture of oligos for diagnostic use provides customers defined specifications and control over manufacturing processes.

Read More>

How Biotin Became a Tool of Molecular Biologists

Biotechnology has an inclination towards co-opting nature’s most useful tools. The naturally occurring, extraordinarily strong bond that forms between avidins and biotin has provided a useful tool that has enabled numerous techniques that would otherwise be impossible.

Read More>

Understanding Mass Spectrometry of Oligonucleotides

Oligonucleotide synthesis is a complex process that requires more than one hundred sequential chemical reactions to make a single, 25-base sequence. Contemporary synthesis chemistry is robust and modern synthesis platforms are reliable and highly automated. Still, each oligonucleotide synthesized at IDT is evaluated for quality before shipping to ensure that the correct sequence was made. The best method available to assess compound identity in a high throughput environment is mass spectrometry (MS).

Read More>

Dithiol Modifier

Modification Highlight: Thiol-modified oligonucleotides are used in attachment chemistry reactions to bind an oligo to a target. Targets are commonly gold, but can also include a variety of fluorescent and nonfluorescent moieties.

Read More>


Get a free, ongoing print or email subscription to the DECODED newsletter by registering online.