TECHVAULT > DECODED ARCHIVE > PIPET TIPS

Genome editing: How stable is my CRISPR RNA:Cas9 RNP complex?

You can safely complex CRISPR RNAs with Cas9 in advance of your experiments and store these RNPs for future use. Store CRISPR RNPs at 4°C for up to 2 weeks, or at –80°C long-term. RNP complexes stored in this way provide the same high level of genome editing as freshly complexed RNPs.

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Successful CRISPR genome editing in hard-to-transfect cells (i.e., Jurkat cells)

Use the conditions presented here for Clone E6-1 Jurkat cells as a starting point for optimization of CRISPR reagent delivery in cell types requiring electroporation.

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Assessing formulas that calculate Tm—do nearest-neighbor parameters matter?

Should programs that predict Tm include nearest neighbor parameters? Learn which factors are critical for Tm calculation accuracy, and which online software takes them into account.

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Designing sgRNAs for CRISPR/Cas9 experiments

Learn how CRISPR crRNA and tracrRNA sequences can be combined into a single sgRNA for simplified use in CRISPR RNA-guided genome editing.

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Could your PCR be affected by contamination?

Learn how to prevent false amplification from DNA contamination.

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