TECHVAULT > DECODED ARCHIVE > PIPET TIPS

Tips for working with gBlocks® Gene Fragments

Working with IDT custom, synthetic dsDNA fragments? Get these tips from our scientists on the best ways to resuspend, quantify, and calculate copy number of gBlocks® Gene Fragments.

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Considerations when adopting published sequences for your own qPCR assay

Published papers that use qPCR applications are a resource of vetted assay sequences. These can be co-opted and converted to more sensitive, double-quenched probes for use in your own experiments. Before ordering, though, go through this checklist to ensure that these sequences will work well in your assays, and consider up-to-date PrimeTime Predesigned qPCR Assays with guaranteed efficiencies of >90%.

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Genome editing tip: A CRISPR RNA annealing step can increase editing efficiency

Looking for ways to increase the genome editing activity in your CRISPR experiments? This quick test suggests that spending a little extra time to anneal CRISPR RNAs will provide improvement.

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Could your PCR be affected by contamination?

Learn how to prevent false amplification from DNA contamination, and how to address contamination when it does occur.

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Do your qPCR assays come with sequence information? They should. Here Is why.

qPCR assays (primer & probe sets) from other suppliers are often provided without sequence information. IDT always gives you the sequences to the oligos you order. And that can be very important. Read why.

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