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TECHVAULT > DECODED ARCHIVE > PIPET TIPS

Calculation Tips for Resuspending and Diluting Nucleic Acids

Easy guidelines for making a 100 uM solution; calculating nmoles, ug, copy number, and concentration; and determining concentration equivalencies

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Sample Preparation for Successful qPCR

Considerations for qPCR sample preparation for obtaining accurate and consistent results.

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Steps for a Successful qPCR Experiment

This article will focus on 5′ nuclease assay design and experimental setup considerations that will assist in obtaining accurate and consistent results.

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Running Agarose and Polyacrylamide Gels

Electrophoresis with agarose and polyacrylamide gels is one of the most widely used tools in molecular biology. Gels provide a simple, low-cost way to separate nucleic acids based on size for quantification and purification.

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Tips for Using BLAST to Locate PCR Primers

Need a quick way to find the location of primers within a gene or the expected size of the resultant PCR product? In this tip we show you how to get this information using BLAST.

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ISSUE ARCHIVE