Do Your qPCR Assays Come With Sequence Information? They Should. Here Is Why.

qPCR assays (primer & probe sets) from other suppliers are often provided without sequence information. IDT always gives you the sequences to the oligos you order. And that can be very important. Read why.

Read More>

Recommended Dye Combinations for Multiplex qPCR

Recommendations for selecting dyes for multiplex qPCR that minimize background and avoid overlap of fluorescent signals. Included is a table of compatible dyes for multiplexing on common qPCR instruments and a list of suggested quenchers.

Read More>

My Oligos Have Arrived; Now What?

Recommendations for resuspension and storage of newly received oligonucleotides.

Read More>

Designing PCR Primers and Probes

General guidelines for designing primers and probes and for choosing target locations for PCR amplification.

Read More>

Calculations: Converting from Nanograms to Copy Number

Calculation often used when creating a qPCR standard curve. Link to free, online tool that will do it for you.

Read More>


Get a free, ongoing print or email subscription to the DECODED newsletter by registering online.