TECHVAULT > DECODED ARCHIVE > PIPET TIPS

NGS Target Capture Custom Panels—Determining Probe Number and Cost

The experimental design options that will determine the number (and cost) of probes required for an NGS target capture panel.

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Do Your qPCR Assays Come With Sequence Information? They Should. Here Is Why.

qPCR assays (primer & probe sets) from other suppliers are often provided without sequence information. IDT always gives you the sequences to the oligos you order. And that can be very important. Read why.

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Recommended Dye Combinations for Multiplex qPCR

Recommendations for selecting dyes for multiplex qPCR that minimize background and avoid overlap of fluorescent signals. Included is a table of compatible dyes for multiplexing on common qPCR instruments and a list of suggested quenchers.

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My Oligos Have Arrived; Now What?

Recommendations for resuspension and storage of newly received oligonucleotides.

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Designing PCR Primers and Probes

General guidelines for designing primers and probes and for choosing target locations for PCR amplification.

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