A recombinant Cas9 enzyme that drastically reduces CRISPR off-target effects

Learn about the Cas9 variant, Alt-R® S.p. HiFi Cas9 Nuclease 3NLS, that greatly reduces off-target cutting events during CRISPR genome editing. At the same time, it maintains the high level of on-target editing efficiency as wild-type Cas9 nuclease.

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Synthetic CpG ODNs activate immune cells through the Toll-like receptor (TLR) pathway

Did you know that synthetic CpG oligodeoxynucleotides (ODNs) can serve as an adjuvant to enhance the immune response of vaccines by mimicking the immune-stimulatory effects of unmethylated bacterial or viral sequences? Read about the 3 classes of CpG ODNs and how their distinct structures are tied to their varied functions. Order these modified oligos from IDT.

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Use of template switching oligos (TS oligos, TSOs) for efficient cDNA library construction

Conventional cDNA construction strategies usually result in an underrepresentation of the 5' ends of cDNA. However, use of a template switching chimeric DNA:RNA oligo and MMLV reverse transcriptase can improve on this. See how this approach, dubbed SMART, makes it possible to efficiently amplify the entire full-length transcript pool, in a completely sequence-independent manner.

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Long, custom RNA oligos—Ultramer® RNA Oligonucleotides

Product spotlight: Use Ultramer® RNA Oligonucleotides to increase specificity and improve performance in a variety of RNA-related applications. Learn more about these high-quality, single-stranded custom oligos, made up to 120 bases.

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qPCR with intercalating dyes: PrimeTime® qPCR Primers

Need qPCR assays to use with intercalating dyes? You can obtain PrimeTime qPCR Primers to detect genes in human, mouse, and rat transcriptomes with intercalating dyes such as SYBR® Green and EvaGreen®. Because our primer only and probe-based assays use the same designs, when you decide to include probes in your assays, you can just request the probes that go along with these same primer sequences.

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