Use of template switching oligos (TS oligos, TSOs) for efficient cDNA library construction

Conventional cDNA construction strategies usually result in an underrepresentation of the 5' ends of cDNA. However, use of a template switching chimeric DNA:RNA oligo and MMLV reverse transcriptase can improve on this. See how this approach, dubbed SMART, makes it possible to efficiently amplify the entire full-length transcript pool, in a completely sequence-independent manner.

Read More>

Long, custom RNA oligos—Ultramer® RNA Oligonucleotides

Product spotlight: Use Ultramer® RNA Oligonucleotides to increase specificity and improve performance in a variety of RNA-related applications. Learn more about these high-quality, single-stranded custom oligos, made up to 120 bases.

Read More>

qPCR with intercalating dyes: PrimeTime® qPCR Primers

Need qPCR assays to use with intercalating dyes? You can obtain PrimeTime qPCR Primers to detect genes in human, mouse, and rat transcriptomes with intercalating dyes such as SYBR® Green and EvaGreen®. Because our primer only and probe-based assays use the same designs, when you decide to include probes in your assays, you can just request the probes that go along with these same primer sequences.

Read More>

Generate codon balanced libraries for mutagenesis with trimer modifications

Incorporating oligo codon trimers into oligo libraries results in balanced encoding of amino acids and eliminates unwanted stop codons. Such oligo libraries are useful for mutagenesis experiments to prepare proteins for screening for potential improvements in biological function.

Read More>

N6-methyladenosine (m6A)—modulating RNA localization, structure, stability, splicing, and translation

Modification Highlight: Read about the mechanism of methylation and functions of N6-methyladenosine (m6A) modifications in the cell. N6-methyladenosine is available as a popular non-catalog modification from IDT, providing a substrate for studying the role of this natural, reversible, post-translational modification.

Read More>


Get a free, ongoing print or email subscription to the DECODED newsletter by registering online.