Design efficient PCR and qPCR primers and probes using online tools

Simplify planning of your qPCR experiments using IDT free, online tools for oligonucleotide analysis and PCR primer design. This article provides an overview of our predesigned qPCR assays and the basics of designing customized PCR primers and hydrolysis probes with the PrimerQuest® Tool.

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PrimerQuest® Tool: From basic to highly customizable designs

Read these tips on how to use the PrimerQuest Tool to customize primers and probe for a wide variety of qPCR applications. Examples show how to adjust reaction conditions, add a probe to a set of previously designed primers, define primer positions, and include or exclude sequences from the assay designs.

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Selecting qPCR assays in plates for high throughput and large-scale studies

Learn some tricks for easy ordering of qPCR assays in plates.

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Codon optimization tool makes synthetic gene design easy

Use the free IDT Codon Optimization Tool to simplify designing synthetic genes and gBlocks® Gene Fragments for expression in a variety of organisms. The tool allows for manual changes, and takes into account natural codon bias and synthesis complexity.

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A Quick Guide to Ordering xGen® Blocking Oligos

Step-by-step guide to selecting and ordering Universal, Standard, and Custom Blocking Oligos. These sets of oligonucleotides bind adapters used in NGS target capture, reducing nonspecific adapter binding. Their use results in improved number of reads on-target and increased depth of enrichment.. Sets of Blocking Oligos are available for specific platform adapter sequences.

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