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TECHVAULT > FAQ > APPLICATION SUPPORT > PCR AND SEQUENCING

Do you have suggestions for design criteria for multiplex primers?

You should design your primers just as you'd design a typical PCR primer: 

  • Keep the length between 18-24nts, keep the G/C content between 40-60%
  • Make sure all of the Tms are within 5C of each other
  • Avoid strong primer interactions by doing selfdimer and heterodimer checks with our OligoAnalyzer.

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