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Can IDT custom-synthesize a dual labeled oligonucleotide (5' FAM/3' TAMRA)?

The final price will depend on the scale and type of TAMRA you choose. We have two types of TAMRA:

NHS-ester chemistry


The oligo is synthesized with an amino modifier on the 3' end and the 5' dye attached using standard cyanoethyl phosphoramidite chemistry. The oligo is then reverse phase HPLC purified to remove truncated and deletion
mutant products. Then the 3' dye is coupled to the amino group of the modifier (thus, "hand tagged") and the oligo is repurified by IE-HPLC to remove uncoupled dye.

CPG Conjugate


The oligo is synthesized using a dye "impregnated" control pore glass. The reagent has an 8 mixed polarity spacer arm. The attachment to the oligo is via the standard cyanoethyl phosphoramidite chemistry and HPLC purified to removed truncated/deletion mutant products and oligos without the fluorescent labels.


The difference is the intensity of the fluorescence and price. In the CPG conjugate form the dye is exposed to acid each time a base is added on. This means that the fluorescent rings could be acid-nicked and opened, thereby decreasing the intensity of the fluorescence. The NHS-ester version, the dye is never exposed to acid and the rings remain intact and fluorescent. The NHS ester version is more expensive than the CPG conjugate.

Full pricing information can be found in the dual labeled probe section of our online catalog.  Also, since the probe is greater than 35 bases in length, there will be an additional 10% fee added to the cost of the oligo to cover the increased difficulty.

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