How do I calculate the molecular weight of an oligo?

The correct calculation for determining molecular weight is to sum the weights of individual bases and subtract the weight of H2PO4 (63.980) and add the weight of two hydrogens (2.016). You may see some slight difference between hand calculation and OligoAnalyzer due to rounding differences. (313.209*3)+(329.208*3)+(289.184*3)+(304.196*3)-63.980+2.016 equals 3645.427.

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