What are the criteria in choosing an amplicon?

Ideal amplicon size could depend on many variables and design preferences. It is unlikely that an amplicon is too short, however amplicons of great length may need extra time to be polymerized during the extension step, you should make sure the polymerization rate of the enzyme being used is sufficient to copy the amplicon in its entirety, you may need to increase your extension cycle time to accommodate large amplicons.

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