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What type of oligo do you recommend for site-directed mutagenesis?

Ultramer™ Oligonucleotides are especially useful for site-directed mutagenesis reactions. Ultramer Oligonucleotides are up to 200 bases long and are synthesized using IDT’s high-fidelity synthesis systems and chemistries. They are the longest, highest quality oligonucleotides commercially available. Incorporating Ultramer Oligonucleotides as the mutagenic primers allows for greater flexibility in the type and size of sequence changes that can be made. Researchers can add long stretches of new sequences to an existing clone by adding up to 180 bases to a 20 base PCR primer, make changes to a large area within a clone in a single reaction, or change or correct multiple sequence locations simultaneously. Ultramer primers also provide a new tool for regions that have traditionally been difficult to target by extending primers into regions with more optimal sequence composition than was previously attainable. 

Mutagenesis will be affected by truncations and deletion mutations in your oligos. Because of this, we suggest using PAGE-purified oligonucleotides for your procedure.

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