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How do I resuspend my PrimeTime® qPCR Assay?

PrimeTime qPCR Assays can be re-suspended as 40X, 20X or 10X stocks. Different concentrations may be preferred depending on the size of the assay and desired final reaction volume. Resuspend the assay in IDTE Buffer (10 mM Tris, 0.1 mM EDTA pH 8.0 -- available from IDT) at the volumes indicated in the chart below. After adding the volume indicated, pipette the solution up and down the sides of the tube to ensure maximal product recovery. Following resuspension, store at -20°C.

 

Final Desired Stock Concentration

40x

20x

10x

PrimeTime® Mini qPCR Assay

 

100 µL

200 µL

PrimeTime® Standard qPCR Assay

250 µL

500 µL

1000 µL

PrimeTime® XL qPCR Assay

1250 µL

 

 

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