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How do I choose the best internal control target for normalizing qPCR results?

IDT recommends that you test at least two, but preferably three, normalizing or housekeeping genes to ensure accurate internal controls. The best normalizing gene to use will depend on the species and conditions of the sample you will be testing. If you are unsure of the best normalizing gene to use, review the literature for the genes tested on samples with conditions similar to yours. Once you have chosen a normalizing gene, be sure to run a reaction to verify that the gene is, in fact, normalized across samples before you use it as a control.

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