For bacterial transformations, IDT recommends using 100–200 pg of plasmid. This amount can be obtained as follows:
- Add 20 µL of TE buffer (pH 7.5–8.0) to the dry plasmid to create a 100 ng/µL stock concentration.
- Add 1 µL of stock to 999µL of water to create a working concentration of 100 pg/µL.
- Use 1–2 µL of the working concentration for the transformation.
The optimal amount of plasmid to be introduced into yeast or mammalian cells lines varies with the cell line and the transfection method; follow the protocol for your transfection reagent.