Which vector is used for standard gene synthesis orders? What restriction sites will be available for subcloning?
- The vector we use for larger genes is pUC57, and the vector we use for minigenes is pIDT-SMART.
- You will need to design your insert with restriction sites of your choice to remove it from the vector. If you order on the web, it is much easier because you can choose the restriction sites from a drop-down menu and the website will automatically add them to your insert.