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Top Questions

Should I amplify my gBlocks® Gene Fragments when I receive them?

gBlocks® Gene Fragments are delivered dried down, normalized to 200 ng, which should provide sufficient DNA for 2–4 cloning reactions according to most protocols. We recommend avoiding amplification of gBlocks Gene Fragments because of the potential for polymerase introduced errors. If you do need to amplify your gBlocks Gene Fragments or require more material than the 200 ng provided, it is strongly recommended that you use a high-fidelity polymerase in order to limit the introduction of errors into your gBlocks Gene Fragments sequence. A protocol for amplification using the Phusion® DNA polymerase (NEB) can be downloaded at www.idtdna.com/gBlocks, under the Support tab. For additional information, download a copy of the gBlocks Gene Fragments user guide or contact us at genes@idtdna.com.
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