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How do I determine nmol product from an oligonucleotide solution (e.g., for oligo normalization specifications)?

Oligo quantity (nmol) is directly proportional to the oligo concentration (µM) and volume (µL).

The calculation for oligo quantity is:

The calculation for oligo concentration is:

The calculation for final volume is:

Tags:
  • plates
  • yield
  • normalizing
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