Top Questions

How do I dilute and store PrimeTime qPCR Probes?

We recommend resuspending PrimeTime qPCR Probes in TE (10mM Tris pH 8.0, 0.1 mM EDTA). Alternatively, sterile dH2O can be used. DNA kept in TE buffer, frozen in a nuclease-free environment should be stable for up to 2 years. We find it convenient to initially make a  at 100 µM freezer stock (which should be thawed relatively infrequently). Adding a volume a TE (µL) equal to 10 x # nanomoles DNA present in the tube (as noted on the spec sheet provided with the oligo) will produce a stock solution at this concentration.
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