Top Questions

Do I need to form my gRNA/RNP complexes separately before multiplexing?

Yes. Forming the crRNA:tracrRNA:Cas9 ribonucleoprotein complex prior to mixing multiple crRNAs helps prevent undesireable interactions between different crRNAs before they have an opportunity to correctly hybridize with tracrRNA.
Tags:
  • Alt-R
  • CRISPR
  • Cas9
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