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How do you recommend assessing off-target effects?

To avoid problems with coverage using in-cell techniques, we recommend initial screening for off-target effects using cell-free methods, such as circle-seq or site-seq. Guide-seq is one unbiased live-cell method to consider. Bioinformatics-based methods for predicting off-target sites are not sufficiently comprehensive.
Tags:
  • CRISPR
  • Cas9
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