Top Questions

How does Alt-R® S.p. HiFi Cas9 Nuclease 3NLS differ from the standard Alt-R S.p. Cas9 Nuclease 3NLS?

Alt-R S.p. HiFi Cas9 Nuclease 3NLS was developed to reduce off-target cutting events, with similar high, on-target activity as the Alt-R S.p. Cas9 Nuclease 3NLS.

Alt-R HiFi Cas9 nuclease can be substituted for Alt-R Cas9 nuclease in experiments that are sensitive to off-target cutting events. We recommend testing a dose-response curve using Alt-R HiFi Cas9 or other Cas9 proteins to determine the optimal dosage.
  • Alt-R
  • Cas9
Application Support Topics