Top Questions

How much fluorescently labeled tracrRNA should I use in my CRISPR-Cas9 experiments?

The amount of Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550 needed varies by application and transfection method. Here are our recommendations. Use these as starting points as you optimize your experiments.

Table. Final concentration of Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550 to use in transfection

 FACS analysisFluorescence microscopy
Lipofection~10 nM10 nM
Electroporation≥0.5 µM
~0.5 µM
Tags:
  • Cas9
  • CRISPR
  • Alt-R
Application Support Topics