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I transfected my cells with the fluorescently labeled Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550. When should I sort my cells by fluorescence activated cell sorting (FACS)?

For optimal results, we recommend sorting your cells by FACS 24 hours after delivery of the ribonucleoprotein (RNP) complex containing the Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550. The optimal signal intensity occurs at 24 hours, after which signal intensity begins to decrease.

Tags:
  • Cas9
  • CRISPR
  • Alt-R
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