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Top Questions

Is it OK to resuspend and store oligos in DEPC treated water?

No, we do not recommend resuspending or storing oligos in DEPC treated water.  DEPC treated water can be acidic which may cause depurination of the oligo resulting in degradation. For optimal stability, oligos that are to be stored long term should be stored frozen, at–20°C.  If the oligos are going to be resuspended, storage in a TE buffer (10 mM Tris pH 8.0, 0.1 mM EDTA; such as IDTE) is better than using water. The impact from the type of storage medium used is marginal at -20°C, but becomes more important at higher temperatures (see Figures 1–3 above). It is ideal to store oligos in the dark. Exposure to UV light should be avoided, and ambient lab light should be minimized, particularly for some types of modified oligos.

Also, avoid unnecessary freeze thaw cycles by preparing a freezer stock solution (100 µM) and several working aliquots.  Use the IDT Resuspension Calculator under the "SciTools®" tab of the IDT website for help determining volumes to use.

Please see the Technical Report, Oligonucleotide Stability Study, for data on oligonucleotide storage and a more thorough explanation.

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