Top Questions

Can the PrimeTime® Gene Expression Master Mix be used with fast cycling conditions?

Yes. The PrimeTime® Gene Expression Master Mix can be used with either standard or fast cycling conditions. The conditions for both are provided here as well as in the PrimeTime® Gene Expression Master Mix protocol:

  • Standard cycling conditions: 3 min. 95°C; 35–45 x (15 sec. 95°C; 1 min. 60°).
  • Fast cycling conditions: 3 min. 95°C; 35–45 x (5 sec. 95°C; 30 sec. 60°).
Tags:
  • qPCR master mix
  • qPCR
Application Support Topics