Top Questions

Does the PrimeTime® Gene Expression Master Mix use a hot-start polymerase?

Yes. The PrimeTime® Gene Expression Master Mix uses an antibody-mediated, hot-start polymerase. During the initial denaturation step of qPCR cycling, polymerase activity is unblocked when the polymerase is released from the antibody. This hot-start enhances specificity and sensitivity of the qPCR by avoiding non-specific amplification of DNA and primer dimer formation. It is therefore critical to follow the cycling conditions provided in the PrimeTime® Gene Expression Master Mix protocol.

Tags:
  • qPCR
  • qPCR master mix
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