Top Questions

What concentrations are the primers and probe in the PrimeTime® qPCR Assay?

All PrimeTime® qPCR Assay primers and probe are shipped dried down. The amount of primer and probe shipped depends on the assay scale ordered.  The Mini scale is available only with probe containing 5' FAM™ dye, internal ZEN™ quencher, and 3' Iowa Black® FQ, and is sent at a fixed amount of 1 nmole primers (each) and 0.5 nmole probe.  The Standard (500-20 µL reactions) and XL (2500-20 µL reactions) Assay scales have options for  primer-to-probe ratio (ranging from 1:1 to 4:1), and a variety of dye/quencher combinations are available.
Tags:
  • quencher
  • dye
  • probe
  • primers
  • primer
  • scale
  • primetime
Application Support Topics