TechVault

Can I order multiple variable regions in my gBlocks Gene Fragment?

Jan 27, 2014, 23:48 PM
Currently, only consecutive bases are allowed. Specifically, up to 18 consecutive N or K bases can be ordered, with a minimum of 125 bp of fixed, flanking sequence on either side of the variable bases. The maximum length for the entire sequence is currently 500 bp, including the variable bases.
Categories:
  • Synthetic Biology
  • Mutagenesis
  • Cloning and Fragment Assembly
  • Cloning
Tags:
  • variable
  • mutagenesis
  • libraries
  • gBlocks
  • gblock