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TechVault

Can I use probes with Iowa Black® FQ or Iowa Black® RQ on Applied Biosystems Real-Time PCR Systems?

Iowa Black® FQ and Iowa Black® RQ labeled probes can be used on any PCR instrument. Setup varies between instruments but, generally, if there is an option for picking a dye name for a quencher, pick "non-fluorescent (NFQ)", "none" or "dark quencher". The same setting used for MGB quenchers may be used for Iowa Black® dark quenchers.
Tags:
  • probe
  • pcr
  • multiplex
  • quencher