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TechVault

Do custom oligos come with 5’ phosphorylation?

Synthetic DNA and RNA are synthesized with 5’ and 3’ OH groups. If your application requires a 5’ phosphate group, such as a ligation reaction, include a phosphorylation modification as part of your sequence. The modification code for 5’ phosphorylation is /5Phos/ and would be inserted into your sequence at the 5’ end.
Tags:
  • phosphorylation
  • ligation
  • ligate