TechVault

Do I need to form my gRNA/RNP complexes separately before multiplexing?

Jul 31, 2017, 06:00 AM
Yes. Forming the crRNA:tracrRNA:Cas9 ribonucleoprotein complex prior to mixing multiple crRNAs helps prevent undesireable interactions between different crRNAs before they have an opportunity to correctly hybridize with tracrRNA.
Categories:
  • Genome Editing
  • Genome Editing
Tags:
  • Alt-R
  • CRISPR
  • Cas9