How can I assemble long, single-stranded oligos to create several kb, clonable gene sequences (assembly PCR)?

May 31, 2015, 04:59 AM

Assembly PCR is a very flexible technique for producing novel gene sequences from single-stranded oligos or a mix of single- and double-stranded DNA. Several single-stranded oligonucleotides, such as the IDT 60–120 nt Ultramer® Oligonucle­otides, can be designed with short overlap­ping sequences and then assembled in a 2-step process to produce longer genes of up to several thousand base pairs. Con­siderations for setting up such reactions and a brief protocol can be found in the article, Assembly PCR for Novel Gene Synthe­sis, in DECODED 2.4, October 2012 ( IDT also offers gBlocks® Gene Fragments, custom synthe­sized, double-stranded DNA fragments that can streamline the assembly and cloning process. Learn more at

  • Synthetic Biology
  • PCR/qPCR
  • gBlocks Gene Fragments
  • Cloning and Fragment Assembly
  • Cloning
  • ultramer
  • synthetic
  • clone
  • assembly