TechVault

How many experiments can I expect to get from a tube of Cas9?

Jul 31, 2017, 06:00 AM

It depends upon your method for delivery, reaction volume, and Cas9 concentration.

As an example:

Tube size Delivery method Reaction volume Cas9 concentration Number of reactions
100 µg Lipofection 150 µL 10 nM ~400
100 µg Electroporation 10 µL 3 µM ~20
Categories:
  • Genome Editing
  • Genome Editing
Tags:
  • Alt-R
  • CRISPR
  • Cas9